HIV-1-stimulated expression of CD11/CD18 integrins and ICAM-1: a possible mechanism for extravascular dissemination of HIV-1-infected cells

Trans Assoc Am Physicians. 1989;102:117-30.


To identify mechanisms which might facilitate emigration of HIV-1-infected cells from the circulation, we studied the effect of HIV-1 infection on T lymphocyte and monocytoid cell expression of molecules involved in adherence and translocation of leukocytes across endothelial cell barriers. CD11a, CD18, and ICAM-1 were demonstrated on up to 80% of HIV-1-infected H9 T cells by flow cytometry; these molecules were not evident on uninfected H9. CD18 mRNA was detected in HIV-infected, but not in uninfected H9 T cells. Cell surface expression of CD11a and CD18, but not ICAM-1, was increased on HIV-infected, as compared to uninfected U937 and THP1 monocytoid cells. Increased cell surface expression of the leukocyte integrins was associated with a significantly increased tendency of HIV-infected monocytoid cells to adhere to human umbilical vein endothelial cell monolayers or aggregate homotypically. Preincubating the monocytoid cells with anti-CD18 or anti-CD11a or preincubating endothelial cells with anti-ICAM-1 suppressed these cell to cell interactions. These studies suggest that HIV-1 infection stimulates cell surface expression of molecules involved in leukocyte adherence and transendothelial migration in vitro. Similar mechanisms may influence leukocyte trafficking, in vivo, and may play a role in the localization of HIV-1 infected cells in the central nervous system and other tissues.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Differentiation / metabolism
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism*
  • Cell Line
  • Endothelium, Vascular / microbiology
  • Endothelium, Vascular / physiopathology
  • HIV Infections / immunology
  • HIV Infections / microbiology
  • HIV Infections / physiopathology*
  • HIV-1 / isolation & purification
  • HIV-1 / physiology*
  • Humans
  • Integrins / metabolism*
  • Intercellular Adhesion Molecule-1
  • Nervous System / immunology
  • Nervous System / microbiology
  • Nervous System / physiopathology
  • Receptors, Leukocyte-Adhesion / metabolism


  • Antigens, Differentiation
  • CD11 Antigens
  • CD18 Antigens
  • Cell Adhesion Molecules
  • Integrins
  • Receptors, Leukocyte-Adhesion
  • Intercellular Adhesion Molecule-1