Frequency and Genotype of Human Parvovirus B19 Among Iranian Patients Infected With HIV

J Med Virol. 2015 Jul;87(7):1124-9. doi: 10.1002/jmv.24169. Epub 2015 Mar 13.

Abstract

The human parvovirus B19 (B19) usually causes a subclinical infection in immunocompetent individuals. Whereas immunocompromised individuals such as patients infected with HIV are at risk of persistent anemia due to B19 infection. Only few studies have been carried out on distribution and molecular epidemiology of B19 in Iran. We aimed to determine the frequency and genotype of B19 among Iranian patients infected with HIV. We conducted a survey on 99 HIV patients and 64 healthy controls. IgG and IgM antibodies against B19 were detected by ELISA and B19 DNA was assessed by nested PCR. PCR products were subjected to direct sequencing and classified after phylogenetic analysis. The prevalence of B19 immunoglobulin was 11.1% for IgG and 1% for IgM. B19 DNA was detected in 13.1% of cases. The prevalence of B19 IgG, IgM, and DNA in control group was 25%, 1.6%, and 9.4%, respectively. B19 IgG was significantly lower in HIV group than in normal controls. There was no significant difference regarding anemia between cases and controls. All sequenced B19 isolates belonged to genotype 1A with low genetic diversity. Our findings indicated that in the HAART era, the importance of B19 infections in HIV patients may be limited whereas persistent B19 viremia in the circulation of healthy controls raises a potential concern in blood donations.

Keywords: anemia; genotype; human immunodeficiency virus (HIV); human parvovirus B19 (B19); serology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Viral / blood
  • Antibodies, Viral / immunology
  • CD4 Lymphocyte Count
  • Case-Control Studies
  • Coinfection*
  • Female
  • Genes, Viral
  • Genotype*
  • HIV Infections / epidemiology*
  • HIV Infections / immunology
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Immunoglobulin M / blood
  • Immunoglobulin M / immunology
  • Iran / epidemiology
  • Male
  • Middle Aged
  • Parvoviridae Infections / epidemiology*
  • Parvoviridae Infections / immunology
  • Parvoviridae Infections / virology*
  • Parvovirus B19, Human / classification
  • Parvovirus B19, Human / genetics*
  • Parvovirus B19, Human / immunology
  • Parvovirus B19, Human / isolation & purification
  • Phylogeny
  • Viral Load

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M