Ryanodine-mediated conversion of STP to LTP is lacking in synaptopodin-deficient mice

Brain Struct Funct. 2016 May;221(4):2393-7. doi: 10.1007/s00429-015-1026-7. Epub 2015 Mar 14.


In previous studies we and others have found that activation of ryanodine receptors (RyRs) facilitate expression of long-term potentiation (LTP) of reactivity to afferent stimulation in hippocampal slices, with a more pronounced action in the ventral hippocampus. We have also been able to link the involvement of synaptopodin (SP), an actin-binding protein, with neuronal plasticity via its interaction with RyRs. To test this link more directly, we have now compared the ability of ryanodine to convert short-term to LTP in hippocampal slices taken from normal and SP-knockout (SPKO) mice. Indeed, SPKO hippocampus expresses lower concentrations of RyRs and in slices of these mice ryanodine is unable to facilitate conversion of short-term to LTP. These observations link functionally SP with calcium stores.

Keywords: Calcium stores; Hippocampus; LTP; Ryanodine receptors; Synaptopodin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Excitatory Postsynaptic Potentials
  • Hippocampus / metabolism
  • Hippocampus / physiology*
  • Long-Term Potentiation* / drug effects
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microfilament Proteins / genetics
  • Microfilament Proteins / physiology*
  • Ryanodine / administration & dosage
  • Ryanodine Receptor Calcium Release Channel / metabolism
  • Ryanodine Receptor Calcium Release Channel / physiology*


  • Microfilament Proteins
  • Ryanodine Receptor Calcium Release Channel
  • Synpo protein, mouse
  • Ryanodine