Direct visualization of newly synthesized target proteins in situ

Nat Methods. 2015 May;12(5):411-4. doi: 10.1038/nmeth.3319. Epub 2015 Mar 16.


Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies
  • Cells, Cultured
  • Fibroblasts / metabolism*
  • Gene Expression Regulation / physiology
  • Hippocampus / cytology
  • Mice
  • Neurons / metabolism
  • Proteins / chemistry*
  • Proteins / metabolism*
  • Rats
  • Staining and Labeling


  • Antibodies
  • Proteins