Structure of a new pseudaminic acid-containing capsular polysaccharide of Acinetobacter baumannii LUH5550 having the KL42 capsule biosynthesis locus

Carbohydr Res. 2015 Apr 30:407:154-7. doi: 10.1016/j.carres.2015.02.006. Epub 2015 Feb 23.

Abstract

The capsular polysaccharide from Acinetobacter baumannii LUH5550 was studied by 1D and 2D (1)H and (13)C NMR spectroscopy. The following structure of the branched trisaccharide repeating unit was established: [structure: see text] where Pse5Ac7RHb indicates 5-acetamido-3,5,7,9-tetradeoxy-7-[(R)-3-hydroxybutanoylamino]-L-glycero-L-manno-non-2-ulosonic acid. The genes in the capsule biosynthesis locus designated KL42 are consistent with the structure established.

Keywords: Acinetobacter baumannii; Capsular polysaccharide structure; Polysaccharide gene locus; Pseudaminic acid.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acinetobacter baumannii / chemistry*
  • Acinetobacter baumannii / genetics
  • Carbohydrate Sequence
  • Hydrolysis
  • Polysaccharides, Bacterial / chemistry
  • Polysaccharides, Bacterial / genetics*
  • Polysaccharides, Bacterial / isolation & purification*

Substances

  • Polysaccharides, Bacterial