Radio-immunoassay of somatostatin from isolated rat pancreatic islets

Scand J Clin Lab Invest. 1989 Apr;49(2):135-8. doi: 10.3109/00365518909105411.

Abstract

Certain aspects of radio-immunoassay of somatostatin from isolated rat pancreatic islets are described. Somatostatin-14, and not somatostatin-28, is secreted from isolated rat pancreatic islets. Less somatostatin secretion is measured per islet owing to purity of tracer in the radio-immunoassay. Theophylline apparently cross-reacts with somatostatin in the assay described, and this has to be taken into consideration when studying somatostatin release induced by theophylline in isolated islets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Glucose / pharmacology
  • Islets of Langerhans / metabolism*
  • Quality Control
  • Radioimmunoassay*
  • Rats
  • Somatostatin / analysis*
  • Somatostatin / metabolism
  • Somatostatin-28
  • Theophylline / analysis
  • Theophylline / pharmacology

Substances

  • Somatostatin
  • Somatostatin-28
  • Theophylline
  • Glucose