Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille

J Biosci Bioeng. 2015 Aug;120(2):181-6. doi: 10.1016/j.jbiosc.2014.12.012. Epub 2015 Mar 14.


Dendropanax morbifera H. Lev. is well known in Korean traditional medicine for improvement of blood circulation. In this study, rutin, a bioflavonoid having anti-thrombotic and anticoagulant activities was isolated from a traditional medicinal plant, D. morbifera H. Lev. The chemical characteristics of rutin was studied to be quercetin 3-O-α-l-rhamnopyranosyl-(1-6)-β-d-glucopyranoside using high performance liquid chromatography mass spectrometry (HPLC-MS), proton nuclear magnetic resonance ((1)H NMR) and carbon-13 nuclear magnetic resonance ((13)C NMR). Turbidity and fibrin clotting studies revealed that rutin reduces fibrin clot in concentration dependent manner. Rutin was found to prolong activated partial thromboplastin time (aPTT), prothrombin time (PT) and closure time (CT). Furthermore, it decreased the activity of pro-coagulant protein, thrombin. In vivo study showed that rutin exerted a significant protective effect against collagen and epinephrine (or thrombin) induced acute thromboembolism in mice. These results suggest that rutin has a potent to be an anti-thrombotic agent for cardiovascular diseases.

Keywords: Antithrombotic; Dendropanax morbifera Leveille; Rutin; Thromboembolism; Thrombosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticoagulants / chemistry
  • Anticoagulants / isolation & purification
  • Anticoagulants / pharmacology
  • Antithrombins / chemistry
  • Antithrombins / isolation & purification*
  • Antithrombins / pharmacology*
  • Araliaceae / chemistry*
  • Blood Coagulation / drug effects
  • Blood Platelets / drug effects
  • Collagen / adverse effects
  • Epinephrine / adverse effects
  • Fibrin / metabolism
  • Male
  • Medicine, Korean Traditional
  • Mice
  • Partial Thromboplastin Time
  • Plants, Medicinal / chemistry*
  • Prothrombin Time
  • Rutin / chemistry
  • Rutin / isolation & purification*
  • Rutin / pharmacology*
  • Thrombin / adverse effects
  • Thrombin / metabolism
  • Thromboembolism / chemically induced
  • Thromboembolism / prevention & control


  • Anticoagulants
  • Antithrombins
  • Rutin
  • Fibrin
  • Collagen
  • Thrombin
  • Epinephrine