Chrysanthemum promotes adipocyte differentiation, adiponectin secretion and glucose uptake

Am J Chin Med. 2015;43(2):255-67. doi: 10.1142/S0192415X15500172. Epub 2015 Mar 17.


The adipose tissue is an endocrine organ, and its endocrine function is closely related to type 2 diabetes. Edible Chrysanthemum morifolium Ramat. (ECM) possesses several biological properties; however, its effect on adipocytes remains unclear. We investigated the effect of the hot water extract of ECM (HW-ECM) on 3T3-L1 adipocytes. HW-ECM enhanced adipocyte differentiation, adiponectin secretion, and glucose uptake in 3T3-L1 cells. It also increased the mRNA levels of peroxisome proliferator-activated receptor γ (PPARγ), a regulator of adipocyte differentiation, adiponectin transcription, and GLUT4 expression. In addition, HW-ECM increased the mRNA levels of CCAAT/enhancer-binding protein-delta (C/EBPδ), which induces PPARγ expression, but not C/EBPβ, during early adipocyte differentiation. These results suggest that HW-ECM enhances adipocyte differentiation, adiponectin secretion, and glucose uptake through C/EBPδ-induced PPARγ expression. These effects of HW-ECM on adipocytes suggest that HW-ECM is potentially beneficial for type 2 diabetes.

Keywords: Adipocyte Differentiation; Adiponectin; C/EBPδ; Chrysanthemum morifolium Ramat; PPARγ.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology*
  • Adipocytes / metabolism*
  • Adiponectin / metabolism*
  • Animals
  • CCAAT-Enhancer-Binding Protein-delta / genetics
  • CCAAT-Enhancer-Binding Protein-delta / metabolism
  • CCAAT-Enhancer-Binding Protein-delta / physiology
  • Cell Differentiation / drug effects*
  • Cell Differentiation / genetics
  • Chrysanthemum / chemistry*
  • Diabetes Mellitus, Type 2 / drug therapy
  • Glucose / metabolism*
  • Hot Temperature
  • Mice
  • PPAR gamma / genetics
  • PPAR gamma / metabolism
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • RNA, Messenger / metabolism
  • Stimulation, Chemical
  • Water


  • Adiponectin
  • PPAR gamma
  • Plant Extracts
  • RNA, Messenger
  • Water
  • CCAAT-Enhancer-Binding Protein-delta
  • Glucose