Genome sequence of Ensifer medicae Di28; an effective N2-fixing microsymbiont of Medicago murex and M. polymorpha

Giovanni Garau; Jason Terpolilli; Yvette Hill; Rui Tian; John Howieson; Lambert Bräu; Lynne Goodwin; James Han; Tbk Reddy; Marcel Huntemann; Amrita Pati; Tanja Woyke; Konstantinos Mavromatis; Victor Markowitz; Natalia Ivanova; Nikos Kyrpides; Wayne Reeve

doi:10.1186/1944-3277-9-4

Genome sequence of Ensifer medicae Di28; an effective N2-fixing microsymbiont of Medicago murex and M. polymorpha

Stand Genomic Sci. 2014 Dec 8:9:4. doi: 10.1186/1944-3277-9-4. eCollection 2014.

Authors

Giovanni Garau¹, Jason Terpolilli², Yvette Hill², Rui Tian², John Howieson², Lambert Bräu³, Lynne Goodwin⁴, James Han⁵, Tbk Reddy⁵, Marcel Huntemann⁵, Amrita Pati⁶, Tanja Woyke⁵, Konstantinos Mavromatis⁶, Victor Markowitz⁶, Natalia Ivanova⁴, Nikos Kyrpides⁷, Wayne Reeve²

Affiliations

¹ Dipartimento di Agraria, S.T.A.A., University of Sassari, Sassari, Italy.
² Centre for Rhizobium Studies, Murdoch University, Murdoch, WA, Australia.
³ School of Life and Environmental Sciences, Deakin University, Deakin, VIC, Australia.
⁴ Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, USA.
⁵ DOE Joint Genome Institute, Walnut Creek, CA, USA.
⁶ Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
⁷ Los Alamos National Laboratory, Bioscience Division, Los Alamos, NM, USA ; Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Ensifer medicae Di28 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago spp. Di28 was isolated in 1998 from a nodule recovered from the roots of M. polymorpha growing in the south east of Sardinia (Italy). Di28 is an effective microsymbiont of the annual forage legumes M. polymorpha and M. murex and is capable of establishing a partially effective symbiotic association with the perennial M. sativa. Here we describe the features of E. medicae Di28, together with genome sequence information and its annotation. The 6,553,624 bp standard draft genome is arranged into 104 scaffolds of 104 contigs containing 6,394 protein-coding genes and 75 RNA-only encoding genes. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Keywords: Alphaproteobacteria; Nitrogen fixation; Rhizobia; Root-nodule bacteria.