Isolation of a pluripotent neural stem cell from the embryonic bovine brain

Int J Mol Sci. 2015 Mar 13;16(3):5990-9. doi: 10.3390/ijms16035990.

Abstract

We recently isolated stem cells derived from the brain of a bovine fetus, utilizing a particular mechanical separation method. After improving our experimental conditions, we obtained neural stem cells using an optimized culture medium system. The cells were expanded, established in continuous cell culture and used for immunofluorescence cytochemistry. RT-PCR showed that embryonic neural stem cells (NSCs) not only expresses the protein Sox2, Nestin but also Pax6, Musashi proteins and were differentiated into the three classical neuronal phenotypes (neurons, astrocytes, and oligodendrocytes).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / cytology
  • Astrocytes / metabolism
  • Cattle
  • Cell Differentiation
  • Cell Separation
  • Cells, Cultured
  • Embryo, Mammalian / cytology
  • Eye Proteins / genetics
  • Eye Proteins / metabolism
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Nestin / genetics
  • Nestin / metabolism
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism
  • Neurons / cytology
  • Neurons / metabolism
  • Oligodendroglia / cytology
  • Oligodendroglia / metabolism
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors / genetics
  • Paired Box Transcription Factors / metabolism
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • SOXB1 Transcription Factors / genetics
  • SOXB1 Transcription Factors / metabolism

Substances

  • Eye Proteins
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • Nestin
  • PAX6 Transcription Factor
  • Paired Box Transcription Factors
  • Repressor Proteins
  • SOXB1 Transcription Factors