Kaempferol-3-O-rutinoside from Afgekia mahidoliae promotes keratinocyte migration through FAK and Rac1 activation

J Nat Med. 2015 Jul;69(3):340-8. doi: 10.1007/s11418-015-0899-3. Epub 2015 Mar 18.


The restoration of the epidermal epithelium through re-epithelialization is a critical process in wound healing. Directed keratinocyte migration to the wound is required, and the retardation of this process may result in a chronic, non-healing wound. The present study contributes to research aiming to identify promising compounds that promote wound healing using a human keratinocyte model. The effects of three kaempferol glycosides from an Afgekia mahidoliae leaf extract, kaempferol-3-O-arabinoside, kaempferol-3-O-glucoside, and kaempferol-3-O-rutinoside, on keratinocyte migration were determined. Interestingly, kaempferol-3-O-rutinoside exhibited a pronounced effect on wound closure in comparison to the parental kaempferol and other glycosides. The mechanism by which kaempferol-3-O-rutinoside enhances cell migration involves the induction of filopodia and lamellipodia formation, increased cellular levels of phosphorylated FAK (Tyr 397) and phosphorylated Akt (Ser 473), and up-regulation of active Rac1-GTP. The data obtained in this study may support the development of this compound for use in wound healing therapies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • Drug Evaluation, Preclinical
  • Fabaceae / chemistry*
  • Focal Adhesion Kinase 1 / metabolism*
  • Humans
  • Kaempferols / pharmacology*
  • Keratinocytes / drug effects
  • Keratinocytes / physiology*
  • Plant Leaves / chemistry
  • Wound Healing
  • rac1 GTP-Binding Protein / metabolism*


  • Kaempferols
  • RAC1 protein, human
  • kaempferol-3-O-rutinoside
  • Focal Adhesion Kinase 1
  • PTK2 protein, human
  • rac1 GTP-Binding Protein