Comparison of the AmpliVue, BD Max System, and illumigene Molecular Assays for Detection of Group B Streptococcus in Antenatal Screening Specimens

Shelley A Miller; Eszter Deak; Romney Humphries

doi:10.1128/JCM.00261-15

Comparison of the AmpliVue, BD Max System, and illumigene Molecular Assays for Detection of Group B Streptococcus in Antenatal Screening Specimens

J Clin Microbiol. 2015 Jun;53(6):1938-41. doi: 10.1128/JCM.00261-15. Epub 2015 Mar 18.

Authors

Shelley A Miller¹, Eszter Deak², Romney Humphries³

Affiliations

¹ Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA.
² Department of Pathology and Laboratory Medicine, Santa Clara Valley Medical Center, San Jose, California, USA.
³ Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, California, USA rhumphries@mednet.ucla.edu.

Abstract

The performances of the AmpliVue, BD Max, and illumigene group B Streptococcus (GBS) nucleic acid amplification tests (NAATs) were compared to that of enriched culture for detection of GBS in antenatal screening specimens. Two hundred specimens were tested simultaneously with the NAATs, following 18 to 24 h of Lim broth enrichment; 15% of specimens were culture positive for GBS, whereas 31.5% were positive by at least one NAAT. All three NAATs were more sensitive (sensitivity, 90.9 to 100%) than culture (sensitivity, 53.6%).

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

DNA, Bacterial / analysis
DNA, Bacterial / genetics
Female
Humans
Molecular Typing / methods*
Nucleic Acid Amplification Techniques
Pregnancy
Pregnancy Complications, Infectious / diagnosis*
Pregnancy Complications, Infectious / microbiology
Prenatal Diagnosis / methods*
Streptococcal Infections / diagnosis*
Streptococcal Infections / microbiology
Streptococcus agalactiae / genetics*

Substances

DNA, Bacterial