Biotechnology. A prudent path forward for genomic engineering and germline gene modification

doi:10.1126/science.aab1028

. 2015 Apr 3;348(6230):36-8.

doi: 10.1126/science.aab1028. Epub 2015 Mar 19.

Biotechnology. A prudent path forward for genomic engineering and germline gene modification

David Baltimore¹, Paul Berg², Michael Botchan³, Dana Carroll⁴, R Alta Charo⁵, George Church⁶, Jacob E Corn⁷, George Q Daley⁸, Jennifer A Doudna⁹, Marsha Fenner⁷, Henry T Greely¹⁰, Martin Jinek¹¹, G Steven Martin¹², Edward Penhoet¹³, Jennifer Puck¹⁴, Samuel H Sternberg¹⁵, Jonathan S Weissman¹⁶, Keith R Yamamoto¹⁷

Affiliations

¹ California Institute of Technology, Mail Code 147-75, Pasadena, CA 91125, USA.
² Stanford University School of Medicine, 291 Campus Drive, Stanford, CA 94305, USA.
³ University of California, Berkeley, 450 Li Ka Shing no. 3370, Berkeley, CA 94720-3370, USA. Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA.
⁴ Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Room 4100, Salt Lake City, UT 84112-5650, USA.
⁵ Department of Medical History and Bioethics, School of Medicine and Public Health, University of Wisconsin Law School, 975 Bascom Mall, Madison, WI 53706, USA.
⁶ Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
⁷ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA.
⁸ Boston Children's Hospital, 300 Longwood Avenue, Karp Family Building, 7th Floor, Boston, MA 02115, USA. Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA.
⁹ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. Departments of Molecular and Cell Biology and Chemistry, Howard Hughes Medical Institute, 731 Stanley Hall, MS 3220, University of California, Berkeley, Berkeley, CA 94720-3220, USA. doudna@berkeley.edu.
¹⁰ Center for Law and the Biosciences, Crown Quadrangle 559 Nathan Abbott Way Stanford, CA 94305-8610, USA.
¹¹ Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
¹² Department of Molecular and Cell Biology, College of Letters and Science, University of California, Berkeley, 210K Durant Hall, Berkeley, CA 94720-2920, USA.
¹³ Alta Partners, One Embarcadero Center, 37th Floor, San Francisco, CA 94111, USA.
¹⁴ Department of Pediatrics UCSF School of Medicine, 513 Parnassus Avenue, San Francisco, CA 94143, USA.
¹⁵ Department of Chemistry, 731 Stanley Hall, MS 3220, University of California, Berkeley, CA 94720-3220, USA.
¹⁶ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, Byers Hall, 1700 4th Street, San Francisco, CA 94158-2330, USA.
¹⁷ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. UCSF School of Medicine, 600 16th Street, San Francisco, CA 94158, USA.

PMID: 25791083
PMCID: PMC4394183
DOI: 10.1126/science.aab1028

Biotechnology. A prudent path forward for genomic engineering and germline gene modification

David Baltimore et al. Science. 2015.

. 2015 Apr 3;348(6230):36-8.

doi: 10.1126/science.aab1028. Epub 2015 Mar 19.

Authors

Affiliations

¹ California Institute of Technology, Mail Code 147-75, Pasadena, CA 91125, USA.
² Stanford University School of Medicine, 291 Campus Drive, Stanford, CA 94305, USA.
³ University of California, Berkeley, 450 Li Ka Shing no. 3370, Berkeley, CA 94720-3370, USA. Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA.
⁴ Department of Biochemistry, University of Utah School of Medicine, 15 North Medical Drive East, Room 4100, Salt Lake City, UT 84112-5650, USA.
⁵ Department of Medical History and Bioethics, School of Medicine and Public Health, University of Wisconsin Law School, 975 Bascom Mall, Madison, WI 53706, USA.
⁶ Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA.
⁷ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA.
⁸ Boston Children's Hospital, 300 Longwood Avenue, Karp Family Building, 7th Floor, Boston, MA 02115, USA. Howard Hughes Medical Institute, 4000 Jones Bridge Road, Chevy Chase, MD 20815, USA.
⁹ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. Departments of Molecular and Cell Biology and Chemistry, Howard Hughes Medical Institute, 731 Stanley Hall, MS 3220, University of California, Berkeley, Berkeley, CA 94720-3220, USA. doudna@berkeley.edu.
¹⁰ Center for Law and the Biosciences, Crown Quadrangle 559 Nathan Abbott Way Stanford, CA 94305-8610, USA.
¹¹ Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
¹² Department of Molecular and Cell Biology, College of Letters and Science, University of California, Berkeley, 210K Durant Hall, Berkeley, CA 94720-2920, USA.
¹³ Alta Partners, One Embarcadero Center, 37th Floor, San Francisco, CA 94111, USA.
¹⁴ Department of Pediatrics UCSF School of Medicine, 513 Parnassus Avenue, San Francisco, CA 94143, USA.
¹⁵ Department of Chemistry, 731 Stanley Hall, MS 3220, University of California, Berkeley, CA 94720-3220, USA.
¹⁶ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, Byers Hall, 1700 4th Street, San Francisco, CA 94158-2330, USA.
¹⁷ Innovative Genomics Initiative, University of California, Berkeley, 188 Li Ka Shing Center, Berkeley, CA 94720-3370, USA. UCSF School of Medicine, 600 16th Street, San Francisco, CA 94158, USA.

PMID: 25791083
PMCID: PMC4394183
DOI: 10.1126/science.aab1028

Abstract

A framework for open discourse on the use of CRISPR-Cas9 technology to manipulate the human genome is urgently needed

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Comment in

Eugenics lurk in the shadow of CRISPR.
Pollack R. Pollack R. Science. 2015 May 22;348(6237):871. doi: 10.1126/science.348.6237.871-a. Science. 2015. PMID: 25999496 No abstract available.
Germline gene therapy: We're ready.
Miller HI. Miller HI. Science. 2015 Jun 19;348(6241):1325. doi: 10.1126/science.348.6241.1325-a. Science. 2015. PMID: 26089502 No abstract available.

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References

1. IGI Forum on Bioethics, Napa, California; this meeting was sponsored by the Innovative Genomics Initiative at the University of California, Berkeley; 24 January 2015; University of California, San Francisco. all the authors, except for GC and MJ, participated in the meeting.
1. Doudna JA, Charpentier E. Science. 2014;346:1258096. Medline. - PubMed
1. Yin H, et al. Nat. Biotechnol. 2014;32:551. Medline. - PMC - PubMed
1. Mali P, et al. Science. 2013;339:823. Medline. - PMC - PubMed
1. Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215. Medline. - PMC - PubMed

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[1] IGI Forum on Bioethics, Napa, California; this meeting was sponsored by the Innovative Genomics Initiative at the University of California, Berkeley; 24 January 2015; University of California, San Francisco. all the authors, except for GC and MJ, participated in the meeting.

[2] IGI Forum on Bioethics, Napa, California; this meeting was sponsored by the Innovative Genomics Initiative at the University of California, Berkeley; 24 January 2015; University of California, San Francisco. all the authors, except for GC and MJ, participated in the meeting.

[3] Doudna JA, Charpentier E. Science. 2014;346:1258096. Medline. - PubMed

[4] Doudna JA, Charpentier E. Science. 2014;346:1258096. Medline. - PubMed

[5] Yin H, et al. Nat. Biotechnol. 2014;32:551. Medline. - PMC - PubMed

[6] Yin H, et al. Nat. Biotechnol. 2014;32:551. Medline. - PMC - PubMed

[7] Mali P, et al. Science. 2013;339:823. Medline. - PMC - PubMed

[8] Mali P, et al. Science. 2013;339:823. Medline. - PMC - PubMed

[9] Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215. Medline. - PMC - PubMed

[10] Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215. Medline. - PMC - PubMed

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Biotechnology. A prudent path forward for genomic engineering and germline gene modification

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Biotechnology. A prudent path forward for genomic engineering and germline gene modification

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