Weighing the proteasome for covalent modifications

Chem Biol. 2015 Mar 19;22(3):315-6. doi: 10.1016/j.chembiol.2015.03.003.


Posttranslational modifications (PTMs) control protein function, but established peptide-based proteomic methods often fail to provide a comprehensive view of PTMs. In this issue of Chemistry & Biology, Gersch et al. describe an efficient combination of chromatographic separation and top-down mass spectrometry that together with an intuitive visualization tool allowed them to screen the proteasome for PTMs and covalently binding inhibitors.

Publication types

  • Comment

MeSH terms

  • Humans
  • Proteasome Endopeptidase Complex / analysis*
  • Proteasome Endopeptidase Complex / metabolism*
  • Proteasome Inhibitors / metabolism*
  • Protein Processing, Post-Translational*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*


  • Proteasome Inhibitors
  • Proteasome Endopeptidase Complex