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. 2015 Aug;39(8):1325-8.
doi: 10.1038/ijo.2015.35. Epub 2015 Mar 23.

Are You Also What Your Mother Eats? Distinct Proteomic Portrait as a Result of Maternal High-Fat Diet in the Cerebral Cortex of the Adult Mouse

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Free PMC article

Are You Also What Your Mother Eats? Distinct Proteomic Portrait as a Result of Maternal High-Fat Diet in the Cerebral Cortex of the Adult Mouse

A Manousopoulou et al. Int J Obes (Lond). .
Free PMC article

Abstract

Epidemiological studies suggest an association between maternal obesity and adverse neurodevelopmental outcomes in offspring. Our aim was to compare the global proteomic portrait in the cerebral cortex between mice born to mothers on a high-fat or control diet who themselves were fed a high-fat or control diet. Male mice born to dams fed a control (C) or high-fat (H) diet 4 weeks before conception and during gestation, and lactation were assigned to either C or H diet at weaning. Mice were killed at 19 weeks and their cerebral cortices were analysed using a two-dimensional liquid chromatography-mass spectrometry methodology. In total, 6 695 proteins were identified (q<0.01), 10% of which were modulated in at least one of the groups relative to controls. In silico analysis revealed that mice clustered based on the diet of the mother and not their own diet and that maternal high-fat diet was significantly associated with response to hypoxia/oxidative stress and apoptosis in the cerebral cortex of the adult offspring. Maternal high-fat diet resulted in distinct endophenotypic changes of the adult offspring cerebral cortex independent of its current diet. The identified proteins could represent novel therapeutic targets for the prevention of neuropathological features resulting from maternal obesity.

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
(a) Proteomics workflow and labelling scheme, (b) Principal component analysis of the iTRAQ ratios of all analysed proteins in the cerebral cortex showing clustering of mice based on the maternal diet and not their current diet, that is, blue dots (CH/CC) clustering separately from red (HH/CC) and green dots (HC/CC) along principal component 1. The sample division along principal component 2 results from dividing each sample by a different control, that is, CC1 (dots in the top) or CC2 (dots in the bottom). (c) Venn diagrams of commonly up- and downregulated proteins in CH, HC and HH mice compared with controls (CC). (d) Hierarchical clustering analysis of modulated proteins: the HC and HH groups have a similar proteomic portrait, which was different from that of CH mice.
Figure 2
Figure 2
Process Network Analysis using MetaCore: significant enrichment for (a) response to hypoxia/oxidative stress (FDR corrected P-value=3.53E-02) and (b) apoptosis/endoplasmic reticulum stress pathway (FDR corrected P-value=1.45E-02) in the cerebral cortex of the adult offspring as a result of maternal high-fat diet. Analysed proteins are denoted with a circle (red=upregulation, blue=downregulation).

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