Synthetic and natural Escherichia coli free lipid A express identical endotoxic activities

Eur J Biochem. 1985 Apr 1;148(1):1-5. doi: 10.1111/j.1432-1033.1985.tb08798.x.


The recently chemically synthesized Escherichia coli lipid A and the natural free lipid A of E. coli were compared with respect to their endotoxic activities in the following test systems: lethal toxicity, pyrogenicity, local Shwartzman reactivity, Limulus amoebocyte lysate gelation capacity, tumour necrotizing activity, B cell mitogenicity, induction of prostaglandin synthesis in macrophages, and antigenic specificity. It was found that synthetic and natural free lipid A exhibit identical activities and are indistinguishable in all tests.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Drug Tolerance
  • Endotoxins*
  • Epitopes
  • Escherichia coli / metabolism*
  • In Vitro Techniques
  • Lethal Dose 50
  • Limulus Test
  • Lipid A / toxicity*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mitogens
  • Necrosis / chemically induced
  • Prostaglandins / biosynthesis
  • Pyrogens
  • Rabbits
  • Sarcoma, Experimental / pathology
  • Shwartzman Phenomenon / etiology


  • Endotoxins
  • Epitopes
  • Lipid A
  • Mitogens
  • Prostaglandins
  • Pyrogens