Antigenic Relationship Between the Alpha-Chain of C3, a Leucocyte-Surface Antigen Involved in the Activation of Phagocytic Cells, and a 50,000 MW B-cell Antigen

Immunology. 1985 Apr;54(4):791-800.

Abstract

A monoclonal antibody, M522, reacting with human monocytes, neutrophils and a proportion of non-adherent PBL in a pattern similar to OKM-1 and anti-Mo-1, and precipitating a dimer of MW 165,000 and 100,000 from neutrophils, was shown to react with C3 at an epitope localized on the alpha-chain of C3. F(ab)2-fragments of M522 stimulate the respiratory burst of neutrophils and monocytes. M522 differs from two monoclonal antibodies against the Mo-1 molecule with respect to the capacity to inhibit the binding of sheep erythrocytes coated with different C3-fragments to C-receptor carrying cells. It inhibited the binding of H-coated particles to B-lymphoid cells and precipitated a 50,000 MW molecule from RAJI cells and tonsil lymphocytes. The results obtained suggest an antigenic relationship between the alpha-chain of C3, the heavy chain of a membrane molecule involved in neutrophil/monocyte activation, and a B-cell molecule of MW 50,000.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, Surface / immunology*
  • B-Lymphocytes / immunology*
  • Binding, Competitive
  • Cells, Cultured
  • Complement C3 / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / immunology
  • Humans
  • Membrane Proteins / immunology
  • Molecular Weight
  • Monocytes / immunology
  • Neutrophils / immunology
  • Rosette Formation

Substances

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Complement C3
  • Epitopes
  • Membrane Proteins