A non-invasive platform for functional characterization of stem-cell-derived cardiomyocytes with applications in cardiotoxicity testing

Stem Cell Reports. 2015 Apr 14;4(4):621-31. doi: 10.1016/j.stemcr.2015.02.007. Epub 2015 Mar 19.


We present a non-invasive method to characterize the function of pluripotent stem-cell-derived cardiomyocytes based on video microscopy and image analysis. The platform, called Pulse, generates automated measurements of beating frequency, beat duration, amplitude, and beat-to-beat variation based on motion analysis of phase-contrast images captured at a fast frame rate. Using Pulse, we demonstrate recapitulation of drug effects in stem-cell-derived cardiomyocytes without the use of exogenous labels and show that our platform can be used for high-throughput cardiotoxicity drug screening and studying physiologically relevant phenotypes.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Calcium / metabolism
  • Calcium Signaling / drug effects
  • Cardiotoxicity
  • Cell Culture Techniques
  • Cell Differentiation*
  • Drug Evaluation, Preclinical / methods*
  • High-Throughput Screening Assays
  • Humans
  • Microscopy, Video
  • Myocytes, Cardiac / cytology*
  • Myocytes, Cardiac / drug effects*
  • Myocytes, Cardiac / metabolism
  • Patch-Clamp Techniques
  • Stem Cells / cytology*


  • Calcium