Highly bactericidal Ag nanoparticle films obtained by cluster beam deposition

Nanomedicine. 2015 Aug;11(6):1417-23. doi: 10.1016/j.nano.2015.02.023. Epub 2015 Mar 21.


The recent emergence of bacterial pathogens resistant to most or all available antibiotics is among the major global public health problems. As indirect transmission through contaminated surfaces is a main route of dissemination for most of such pathogens, the implementation of effective antimicrobial surfaces has been advocated as a promising approach for their containment, especially in the hospital settings. However, traditional wet synthesis methods of nanoparticle-based antimicrobial materials leave a number of key points open for metal surfaces: such as adhesion to the surface and nanoparticle coalescence. Here we demonstrate an alternative route, i.e. supersonic cluster beam deposition, to obtain antimicrobial Ag nanoparticle films deposited directly on surfaces. The synthesized films are simple to produce with controlled density and thickness, are stable over time, and are shown to be highly bactericidal against major Gram positive and Gram negative bacterial pathogens, including extensively drug-resistant strains.

From the clinical editor: The use of silver nanoparticle in health care is getting more widespread. The authors here describe the technique of cluster beam deposition for spraying silver on surfaces used in health care sectors. This may open a new avenue for future anti-bacterial coatings.

Keywords: Ag nanoparticle-based antimicrobial films; Atomic force microscopy; Bactericidal activity; Electron spectroscopies; Extensively drug-resistant bacteria; Supersonic cluster beam deposition.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Metal Nanoparticles*
  • Microbial Sensitivity Tests
  • Microscopy, Atomic Force
  • Silver / chemistry
  • Silver / pharmacology*


  • Anti-Bacterial Agents
  • Silver