Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation

Huicong Kang; Qi Hu; Xiaoyan Liu; Yinhe Liu; Feng Xu; Xiang Li; Suiqiang Zhu

doi:10.3969/j.issn.1673-5374.2012.04.002

Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation

Neural Regen Res. 2012 Feb 5;7(4):251-5. doi: 10.3969/j.issn.1673-5374.2012.04.002.

Authors

Huicong Kang¹, Qi Hu¹, Xiaoyan Liu¹, Yinhe Liu², Feng Xu¹, Xiang Li¹, Suiqiang Zhu¹

Affiliations

¹ Department of Neurology, Tongji Hospital of Tongji Medical University, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
² Ministry of Water Resources of China, Beijing 100000, China.

PMID: 25806064
PMCID: PMC4353095
DOI: 10.3969/j.issn.1673-5374.2012.04.002

Abstract

In the present study, we transplanted bone marrow-derived mesenchymal stem cells into the CA3 area of the hippocampus of chronic epilepsy rats kindled by lithium chloride-pilocarpine. Immunofluorescence and western blotting revealed an increase in adenosine A1 receptor expression and a decrease in adenosine A2a receptor expression in the brain tissues of epileptic rats 3 months after transplantation. Moreover, the imbalance in the A1 adenosine receptor/A2a adenosine receptor ratio was improved. Electroencephalograms showed that frequency and amplitude of spikes in the hippocampus and frontal lobe were reduced. These results suggested that mesenchymal stem cell transplantation can reconstruct the normal function of the adenosine system in the brain and greatly improve epileptiform discharges.

Keywords: adenosine system; bone marrow-derived mesenchymal stem cells; cell transplantation; chronic epilepsy; electroencephalogram; immunohistochemistry; reconstruction.