Modulation of satellite cells activity and MyoD in rat thyroarytenoid muscle after reinnervation

Laryngoscope. 2015 Jul;125(7):E245-51. doi: 10.1002/lary.25248. Epub 2015 Mar 26.


Objectives/hypothesis: To examine modulation of M-cadherin, a marker for satellite cells (SCs); and MyoD, which may indicate the myogenic activity following recurrent laryngeal nerve (RLN) denervation and immediate reinnervation; and to elucidate the correlation between their modulations and establishment of neuromuscular junctions (NMJs) in the reinnervated rat thyroarytenoid (TA) muscle.

Study design: Quantitative real-time polymerase chain reaction qPCR and histologic assessment of the TA muscle following RLN transection and anastomosis.

Methods: Rats were divided into three groups: 1) denervation alone (DNV) (n = 60), 2) denervation with anastomosis (ANS) (n = 60), and 3) sham-operated controls (n = 12). Animals were sacrificed at 3 days and 1, 3, and 5 weeks after treatment. TA muscles harvested from 40 animals from each DNV and ANS group; all of sham group were subjected to qPCR for assessment of the modulation of M-cadherin and MyoD; and the remaining larynges of DNV and ANS group were used for histologic analysis.

Results: The expression levels of messenger RNAs (mRNAs) encoding M-cadherin and MyoD in the TA muscle of the DNV group were significantly higher (P < 0.05) than in the control throughout the study period. These mRNA levels in the ANS group were significantly higher (P < 0.05) at ≤ 1 week than in the controls but fell to control levels at ≥ 3 weeks. In the ANS group, recovery of muscle area and NMJs structure occurred by 3 weeks.

Conclusion: These data suggested that NMJ formation following reinnervation might prompt recovery of M-cadherin and MyoD mRNA expression to the quiescent level of SCs.

Keywords: MyoD; Satellite cells; recurrent laryngeal nerve; reinnervation; thyroarytenoid muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / metabolism*
  • Laryngeal Muscles / innervation*
  • Laryngeal Muscles / metabolism
  • Larynx / surgery*
  • Male
  • Muscle Denervation
  • MyoD Protein / metabolism*
  • Neuromuscular Junction / physiology*
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction
  • Recurrent Laryngeal Nerve / surgery*
  • Recurrent Laryngeal Nerve Injuries
  • Satellite Cells, Skeletal Muscle / metabolism*


  • Cadherins
  • MyoD Protein
  • M-cadherin