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. 2015 Feb 1;7(1):e126-32.
doi: 10.4317/jced.52093. eCollection 2015 Feb.

The Intraoral Device of Overlaid Disk-Holding Splints as a New in Situ Oral Biofilm Model

Free PMC article

The Intraoral Device of Overlaid Disk-Holding Splints as a New in Situ Oral Biofilm Model

Isabel Prada-López et al. J Clin Exp Dent. .
Free PMC article


Objectives: To design a device that allows the formation of in situ oral biofilm with similar characteristics to those from the dental plaque, overcoming the limitations of previous devices.

Study design: The Intraoral Device of Overlaid Disk-holding Splints (IDODS) was designed and manufactured. To test its validity, five healthy adult volunteers wore them for two and four days allowing the biofilm to grow without any type of distortion. After each period, the thickness, vitality and structure of the formed biofilm were measured with a Confocal Laser Scanning Microscope (CLSM) in combination with a dual fluorescence solution. All volunteers filled out a Likert-type questionnaire to evaluate the device.

Results: Mean bacterial vitality in the 2- and 4-day biofilms was 71% and 63%, respectively. Mean thicknesses were 21 µm and 28 µm, respectively. There was predominance in the open and heterogeneous structure whose complexity was ascending as the biofilm matured. The results obtained from the questionnaire were 2/5 in the influence in aesthetics, 3.4/5 in comfort, and 5/5 in ease of maintaining oral hygiene and withdrawal from the oral cavity.

Conclusions: A biofilm with optimum characteristics was obtained by IDODS. Its use is associated with good aesthetic and comfort results and is absent of functional limitations, allowing optimal oral hygiene without altering the structure of the in situ oral biofilm. Key words:Confocal Laser Scanning Microscope, fluorochromes, in situ, intraoral device, oral biofilm.

Conflict of interest statement

Conflict of interest statement: The authors declare no conflict of interest.


Figure 1
Figure 1
Sequence of fabrication of the Intraoral Device of Overlaid Disk-holding Splints (IDODS).
Figure 2
Figure 2
Clinical images of the “split-mouth” design of the IDODS. Note that the lower incisors are kept uncovered to improve aesthetics and comfort.
Figure 3
Figure 3
Visualization of channels (yellow stars) and voids (white stars) in different single cross sectional planes from X, Y and Z axis. Images obtained by the CLSM representing different types of biofilm structures: A) The presence of both channels and bubbles shows a heterogeneous architecture model; B) Fluid filled channels; C) Bubble-like structures.

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    1. Auschill TM, Hein N, Hellwig E, Follo M, Sculean A, Arweiler NB. Effect of two antimicrobial agents on early in situ biofilm formation. J Clin Periodontol. 2005;32:147–52. - PubMed
    1. Auschill TM, Hellwig E, Sculean A, Hein N, Arweiler NB. Impact of the intraoral location on the rate of biofilm growth. Clin Oral Investig. 2004;8:97–101. - PubMed
    1. Al-Ahmad A, Wunder A, Auschill TM, Follo M, Braun G, Hellwig E. The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium nucleatum and Veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization. J Med Microbiol. 2007;56:681–7. - PubMed
    1. Al-Ahmad A, Roth D, Wolkewitz M, Wiedmann-Al-Ahmad M, Follo M, Ratka-Kruger P. Change in diet and oral hygiene over an 8-week period: effects on oral health and oral biofilm. Clin Oral Investig. 2010;14:391–6. - PubMed
    1. Wood SR, Kirkham J, Marsh PD, Shore RC, Nattress B, Robinson C. Architecture of intact natural human plaque biofilms studied by confocal laser scanning microscopy. J Dent Res. 2000;79:21–7. - PubMed

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