Isolation of duplicated human c-src genes located on chromosomes 1 and 20

Mol Cell Biol. 1985 Apr;5(4):831-8. doi: 10.1128/mcb.5.4.831.

Abstract

The oncogene (v-src) of Rous sarcoma virus apparently arose by transduction of the chicken gene known as c-src(chicken). We isolated DNA fragments representative of two src-related loci from recombinant DNA bacteriophage libraries of the human genome. One of these loci, c-src1(human), appeared to direct the synthesis of a 5-kilobase polyadenylated RNA that presumably encodes pp60c-src(human). Probes specific for the other locus, c-src2(human), did not hybridize to polyadenylated RNA prepared from a variety of human cell lines. Partial nucleotide sequence determinations of the loci demonstrated that c-src1(human) is highly related to chicken c-src and that c-src2(human) is slightly more divergent. The sequences imply that the final two coding exons of each human locus are identical in length to those of chicken c-src and that the location of an amber stop codon is unchanged in all three loci. c-src1(human) has been mapped to chromosome 20, and the second locus is located on chromosome 1. We conclude that c-src1(human) is the analog of c-src(chicken) and that the duplicated locus, c-src2(human), may also be expressed.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Chickens
  • Chromosome Mapping
  • Chromosomes, Human, 1-3*
  • Chromosomes, Human, 19-20*
  • Cloning, Molecular
  • Gene Expression Regulation
  • Genes
  • Humans
  • Oncogenes*
  • Phosphoproteins / genetics*
  • Protein Kinases / genetics*
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins pp60(c-src)

Substances

  • Phosphoproteins
  • Protein Kinases
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins pp60(c-src)

Associated data

  • GENBANK/K03216
  • GENBANK/K03217
  • GENBANK/K03218
  • GENBANK/K03219