Optimization of covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports for cellulose hydrolysis, in buffer and ionic liquids/buffer media

Artif Cells Nanomed Biotechnol. 2016 Aug;44(5):1276-84. doi: 10.3109/21691401.2015.1024842. Epub 2015 Mar 26.

Abstract

The covalent immobilization of Trichoderma reesei cellulase onto modified ReliZyme HA403 and Sepabeads EC-EP supports were carried out. The optimal immobilization conditions were determined using response surface methodology. The hydrolysis of cellulose using the free and immobilized cellulase preparations in ionic liquids (IL) using cosolvents was investigated. The hydrolytic activities in buffer medium containing 25% (v/v) of 1-butyl-3-methylimidazolium hexafluorophosphate were around 2.6-, 1.6-, and 5.5-fold higher than the activities in buffer medium. The retained initial activities were 32% and 57%, respectively for cellulase preparations immobilized onto Sepabeads EC-EP support and onto modified ReliZyme HA403 support after 5 reuses.

Keywords: ReliZyme HA403; Sepabeads EC-EP; ionic liquids; response surface methodology.

MeSH terms

  • Cellulase / chemistry*
  • Cellulose / chemistry*
  • Enzymes, Immobilized / chemistry
  • Fungal Proteins / chemistry*
  • Hydrolysis
  • Trichoderma / enzymology*

Substances

  • Enzymes, Immobilized
  • Fungal Proteins
  • Cellulose
  • Cellulase