Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA

Chem Commun (Camb). 2015 Apr 28;51(33):7242-4. doi: 10.1039/c5cc00742a.


Using a G-quadruplex bait, we identified the transcription co-activator Sub1 as a G-quadruplex binding protein by quantitative LC-MS/MS and demonstrated in vivo G-quadruplex binding by ChIP. In vitro, Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes. This provides a possible mechanism by which G-quadruplexes can influence gene transcription.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Base Sequence
  • DNA / chemistry*
  • DNA / genetics
  • DNA / metabolism*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism*
  • G-Quadruplexes*
  • Humans
  • Protein Binding
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sequence Homology, Amino Acid*
  • Substrate Specificity
  • Transcription Factors / chemistry
  • Transcription Factors / metabolism*


  • DNA-Binding Proteins
  • SUB1 protein, S cerevisiae
  • SUB1 protein, human
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • DNA