The Effect of Impaired Angiogenesis on Intestinal Function Following Massive Small Bowel Resection

J Pediatr Surg. 2015 Jun;50(6):948-53. doi: 10.1016/j.jpedsurg.2015.03.014. Epub 2015 Mar 14.

Abstract

Purpose: Intestinal adaptation involves villus lengthening, crypt deepening, and increased capillary density following small bowel resection (SBR). Mice lacking the proangiogenic chemokine CXCL5 have normal structural adaptation but impaired angiogenesis. This work evaluates the impact of incomplete adaptive angiogenesis on the functional capacity of the intestine after SBR.

Methods: CXCL5 knockout (KO) and C57BL/6 wild-type (WT) mice underwent 50% SBR. Magnetic resonance imaging measured weekly body composition. Intestinal absorptive capacity was evaluated through fecal fat analysis. Gene expression profiles for select macronutrient transporters were measured via RT-PCR. Postoperative crypt and villus measurements were assessed for structural adaptation. Submucosal capillary density was measured through CD31 immunohistochemistry.

Results: Comparable postoperative weight gain occurred initially. Diminished weight gain, impaired fat absorption, and elevated steatorrhea occurred in KO mice after instituting high-fat diet. Greater postoperative upregulation of ABCA1 fat transporter occurred in WT mice, while PEPT1 protein transporter was significantly downregulated in KO mice. KO mice had impaired angiogenesis but intact structural adaptation.

Conclusion: After SBR, KO mice display an inefficient intestinal absorption profile with perturbed macronutrient transporter expression, impaired fat absorption, and slower postoperative weight gain. In addition to longer villi and deeper crypts, an intact angiogenic response may be required to achieve functional adaptation to SBR.

Keywords: Angiogenesis; CXCL5; Functional intestinal adaptation; High-fat diet; Small bowel resection.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological*
  • Animals
  • Chemokine CXCL5
  • Intestinal Absorption
  • Intestinal Mucosa / pathology
  • Intestine, Small / blood supply*
  • Intestine, Small / physiology
  • Intestine, Small / surgery*
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Neovascularization, Physiologic*

Substances

  • Chemokine CXCL5