Evaluation of the protective efficacy of four novel identified membrane associated proteins of Streptococcus suis serotype 2

Vaccine. 2015 May 5;33(19):2254-2260. doi: 10.1016/j.vaccine.2015.03.038. Epub 2015 Mar 25.

Abstract

Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that can also cause epidemics of life-threatening infections in humans. Surface proteins of pathogens play a critical role in the interaction with host system or environment, as they take part in processes like virulence, cytotoxicity, adhesion, signaling or transport, etc. Thus, surface proteins identified by the screening of immunoproteomic techniques are promising vaccine candidates or diagnostic markers. In this study, four membrane associated proteins (MAP) identified by immunoproteomic method were cloned and expressed as recombinant proteins with his-tag. Screening for vaccine candidates were firstly performed by protection assay in vivo and immunization with Sbp markedly protected mice against systemic S. suis 2 infection. The immune responses and protective of Sbp were further evaluated. The results showed that Sbp could elicit a strong humoral antibody response and protect mice from lethal challenge with S. suis 2. The antiserum against Sbp could efficiently impede survival of bacterial in whole blood killing assay and conferred significant protection against S. suis 2 infection in passive immunization assays. The findings indicate that Sbp may serve as an important factor in the pathogenesis of S. suis 2 and would be a promising subunit vaccine candidate.

Keywords: Membrane associated proteins; Protective antigen; Streptococcus suis serotype 2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / genetics
  • Antigens, Bacterial / immunology*
  • Cloning, Molecular
  • Disease Models, Animal
  • Female
  • Gene Expression
  • Membrane Proteins / genetics
  • Membrane Proteins / immunology*
  • Mice, Inbred BALB C
  • Proteomics
  • Serogroup
  • Streptococcal Infections / immunology
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / prevention & control*
  • Streptococcal Vaccines / administration & dosage
  • Streptococcal Vaccines / genetics
  • Streptococcal Vaccines / immunology*
  • Streptococcal Vaccines / isolation & purification
  • Streptococcus suis / classification
  • Streptococcus suis / genetics
  • Streptococcus suis / immunology*
  • Survival Analysis
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Vaccines, Synthetic / isolation & purification

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Membrane Proteins
  • Streptococcal Vaccines
  • Vaccines, Synthetic