Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities

J Sci Food Agric. 2015 Sep;95(12):2554-61. doi: 10.1002/jsfa.7185. Epub 2015 Apr 10.


Background: Pineapple has a delicious taste and good health benefits. Bioactive polysaccharides are important components of pineapple that might contribute to its health benefits. Since little structural information on these polysaccharides is currently available, the aim of this study was to investigate their structural characteristics and bioactivities.

Results: The polysaccharides of pineapple pulp were fractionated into three fractions (PAPs 1-3) by anion exchange chromatography. Their structural characteristics were first identified, including molecular weights and glycosidic linkages. The monosaccharide compositions were revealed as PAP 1 (Ara, Xyl, Man, Glc and Gal), PAP 2 (Rha, Ara, Xyl, Man, Glc and Gal) and PAP 3 (Rha, Ara, Xyl, Man and Gal). Nuclear magnetic resonance (NMR) spectra suggested that PAP 2 had a backbone of → 4)-α-d-Manp-(1 → 2,4)-α-d-Manp-(1 → with branches attached to O-4 of Manp. The NMR data of α-l-Araf-(1→, →3)-α-l-Araf-(1→, →4)-β-d-Galp-(1 → and → 4)-α-d-GalpAMe-(1 → were assigned. PAPs 1 and 2 showed significant antitumor cell proliferation activities against breast carcinoma cell line and strong antioxidant activities.

Conclusion: The above findings indicated that PAPs 1-3 contributed much to the health benefits of pineapple. They could be used as health-beneficial food additives in functional foods.

Keywords: NMR; antitumor cell proliferation activity; pineapple pulp; polysaccharide; structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ananas*
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Biphenyl Compounds / chemistry
  • Breast Neoplasms / drug therapy
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Female
  • Humans
  • Magnetic Resonance Spectroscopy
  • Pancreatitis-Associated Proteins
  • Phytotherapy*
  • Picrates / chemistry
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Polysaccharides / therapeutic use


  • Antineoplastic Agents
  • Biphenyl Compounds
  • Pancreatitis-Associated Proteins
  • Picrates
  • Polysaccharides
  • REG3A protein, human
  • 1,1-diphenyl-2-picrylhydrazyl