Robust Enumeration of Cell Subsets From Tissue Expression Profiles

Nat Methods. 2015 May;12(5):453-7. doi: 10.1038/nmeth.3337. Epub 2015 Mar 30.

Abstract

We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu/).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers
  • Gene Expression Regulation / physiology
  • Humans
  • Palatine Tonsil / cytology*
  • Palatine Tonsil / metabolism*
  • RNA / classification
  • RNA / genetics
  • RNA / metabolism
  • Reproducibility of Results
  • Software
  • Tissue Culture Techniques / methods*
  • Tissue Preservation / methods*
  • Transcriptome*

Substances

  • Biomarkers
  • RNA

Associated data

  • GEO/GSE65136