An in vitro bioassay for the quantitative evaluation of mosquito repellents against Stegomyia aegypti (=Aedes aegypti) mosquitoes using a novel cocktail meal

Med Vet Entomol. 2015 Sep;29(3):238-44. doi: 10.1111/mve.12114. Epub 2015 Apr 1.


To assess the efficacy of new insect repellents, an efficient and safe in vitro bioassay system using a multiple-membrane blood-feeding device and a cocktail meal was developed. The multiple-membrane blood-feeding device facilitates the identification of new insect repellents by the high-throughput screening of candidate chemicals. A cocktail meal was developed as a replacement for blood for feeding females of Stegomyia aegypti (=Aedes aegypti) (L.) (Diptera: Culicidae). The cocktail meal consisted of a mixture of salt, albumin and dextrose, to which adenosine triphosphate was added to induce engorging. Feeding rates of St. aegypti on the cocktail meal and pig blood, respectively, did not differ significantly, but were significantly higher than the feeding rate on citrate phosphate dextrose-adenine 1 (CPDA-1) solutions, which had been used to replace bloodmeals in previous repellent assays. Dose-dependent biting inhibition rates were analysed using probit analysis. The RD(50) (the dose producing 50% repellence of mosquito feeding) values of DEET, citronella, carvacrol, geraniol, eugenol and thymol were 1.62, 14.40, 22.51, 23.29, 23.83 and 68.05 µg/cm(2), respectively.

Keywords: DEET; Stegomyia aegypti; in vitro bioassay; plant compound; quantitative evaluation; repellent.

MeSH terms

  • Aedes / drug effects*
  • Aedes / physiology
  • Animals
  • Biological Assay / instrumentation
  • Biological Assay / methods*
  • Feeding Behavior / drug effects
  • Female
  • Insect Repellents / pharmacology*


  • Insect Repellents