Reference Standards for Flow Cytometry and Application in Comparative Studies of Nuclear DNA Content

Cytometry. 1989 Nov;10(6):706-10. doi: 10.1002/cyto.990100606.

Abstract

Nuclear DNA mass in cells from a reference species can be used to obtain high-resolution estimates of DNA mass from a target species. In our study of DNA mass in cells from 45 selected species, representing each of the major vertebrate classes, we have obtained values of from 1.5 to 110.0 pg of DNA. Because values in or near this range would be expected in the study of nuclear DNA mass in vertebrates and other organisms, the species in this report can provide a useful catalogue of references for comparative studies of DNA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Nucleus / analysis*
  • DNA / analysis*
  • DNA / standards
  • Flow Cytometry / standards*
  • Reference Standards*
  • Species Specificity
  • Vertebrates

Substances

  • DNA