Tracking by flow cytometry antigen-specific follicular helper T cells in wild-type animals after protein vaccination

Methods Mol Biol. 2015;1291:39-47. doi: 10.1007/978-1-4939-2498-1_4.

Abstract

Flow cytometry is a valuable technology used in immunology to characterize and enumerate the different cell subpopulations specific for a nonself-antigen in the context of an ongoing immune response. Among them, follicular helper T cells are the cognate regulators of B cells in secondary lymphoid tissues. Thus, tracking them is of high interest especially in the context of protein vaccination. For this purpose, transgenic antigen-receptor mouse models have been largely used. It is now clear that transgenic models are not always the best means to study the dynamics of the immune response since they can modify the response. In this chapter, we describe how to track endogenous antigen-specific follicular helper T cells by flow cytometry after protein vaccination in nonmodified wild-type animals, which ultimately provides a comprehensive way to enumerate, characterize, and isolate these particular cells in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anesthesia
  • Animals
  • Cell Count
  • Cell Tracking / methods*
  • Flow Cytometry / methods*
  • Lymph Nodes / cytology
  • Mice, Inbred C57BL
  • Solutions
  • Staining and Labeling
  • Suspensions
  • T-Lymphocytes, Helper-Inducer / cytology*
  • Vaccination*

Substances

  • Solutions
  • Suspensions