Influence of sodium salicylate on rosmarinic acid, carnosol and carnosic acid accumulation by Salvia officinalis L. shoots grown in vitro

Biotechnol Lett. 2015 Aug;37(8):1693-701. doi: 10.1007/s10529-015-1825-1. Epub 2015 Apr 3.

Abstract

Objectives: To evaluate sodium salicylate (NaSA) as an elicitor of rosmarinic acid (RA) and phenolic diterpenes, carnosol (C) and carnosic acid (CA) production, in a culture of Salvia officinalis shoots.

Results: In sage shoots grown in vitro, 28 polyphenolic compounds (phenolic acids, flavonoids, and phenolic diterpenes) were identified. In shoots treated for 1 week with increasing NaSA concentrations, the content of C increased from 2.3 in control to 5.7 mg g(-1) DW in shoots treated with 500 µM NaSA. In shoots that were recovered on basal medium for 3 weeks, the maximal amount of C (14 mg/g(-1) DW) was with 150 µM NaSA treatment. In treated and recovered shoots, the increase in C was accompanied with a decrease in CA, resulting in 1.9-fold increase in the C/CA ratio. Accumulation of RA was not affected by the NaSA treatment. However, elicitation by NaSA was accompanied with growth retardation.

Conclusions: NaSA can improve C production in sage shoot culture, probably by stimulating the conversion of CA to C.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abietanes / metabolism*
  • Cinnamates / metabolism*
  • Culture Media / chemistry
  • Depsides / metabolism*
  • Plant Development / drug effects
  • Plant Shoots / drug effects*
  • Plant Shoots / growth & development
  • Plant Shoots / metabolism
  • Rosmarinic Acid
  • Salvia officinalis / drug effects*
  • Salvia officinalis / growth & development
  • Salvia officinalis / metabolism
  • Sodium Salicylate / metabolism*

Substances

  • Abietanes
  • Cinnamates
  • Culture Media
  • Depsides
  • carnosol
  • salvin
  • Sodium Salicylate