Enhanced Production of Resveratrol, Piceatannol, Arachidin-1, and Arachidin-3 in Hairy Root Cultures of Peanut Co-treated with Methyl Jasmonate and Cyclodextrin

J Agric Food Chem. 2015 Apr 22;63(15):3942-50. doi: 10.1021/jf5050266. Epub 2015 Apr 10.


Peanut (Arachis hypogaea) produces stilbenoids upon exposure to abiotic and biotic stresses. Among these compounds, the prenylated stilbenoids arachidin-1 and arachidin-3 have shown diverse biological activities with potential applications in human health. These compounds exhibit higher or novel biological activities in vitro when compared to their nonprenylated analogues piceatannol and resveratrol, respectively. However, assessment of these bioactivities in vivo has been challenging because of their limited availability. In this study, hairy root cultures of peanut were induced to produce stilbenoids upon treatment with elicitors. Co-treatment with 100 μM methyl jasmonate (MeJA) and 9 g/L methyl-β-cyclodextrin (CD) led to sustained high levels of resveratrol, piceatannol, arachidin-1, and arachidin-3 in the culture medium when compared to other elicitor treatments. The average yields of arachidin-1 and arachidin-3 were 56 and 148 mg/L, respectively, after co-treatment with MeJA and CD. Furthermore, MeJA and CD had a synergistic effect on resveratrol synthase gene expression, which could explain the higher yield of resveratrol when compared to treatment with either MeJA or CD alone. Peanut hairy root cultures were shown to be a controlled and sustainable axenic system for the production of the diverse types of biologically active stilbenoids.

Keywords: Arachis hypogaea; arachidin-1; arachidin-3; cyclodextrin; hairy roots; methyl jasmonate; peanut; piceatannol; resveratrol; stilbenoids.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetates / pharmacology*
  • Arachis / chemistry
  • Arachis / drug effects*
  • Arachis / growth & development
  • Arachis / metabolism
  • Cell Culture Techniques
  • Culture Media / metabolism
  • Cyclopentanes / pharmacology*
  • Gene Expression Regulation, Plant / drug effects
  • Hemiterpenes / analysis
  • Hemiterpenes / metabolism*
  • Oxylipins / pharmacology*
  • Plant Growth Regulators / pharmacology*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plant Roots / drug effects*
  • Plant Roots / genetics
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Resveratrol
  • Stilbenes / analysis
  • Stilbenes / metabolism*
  • beta-Cyclodextrins / pharmacology


  • Acetates
  • Culture Media
  • Cyclopentanes
  • Hemiterpenes
  • Oxylipins
  • Plant Growth Regulators
  • Plant Proteins
  • Stilbenes
  • arachidin-1
  • arachidin-3
  • beta-Cyclodextrins
  • methyl-beta-cyclodextrin
  • 3,3',4,5'-tetrahydroxystilbene
  • methyl jasmonate
  • Resveratrol