Abstract
Pine needle oil from crude extract of pine needles has been used as an anti-cancer agent in Traditional Chinese Medicine. The α-pinene is a natural compound isolated from pine needle oil which has been shown anti-cancer activity. In previous study, we found that pine needle oil exhibited significant inhibitory effect on hepatoma carcinoma BEL-7402 cells. In this study, we investigate the inhibition of α-pinene on hepatoma carcinoma BEL-7402 cells in vitro and in vivo and further explore the mechanism. The results show that liver cancer cell growth was inhibited obviously with inhibitory rate of 79.3% in vitro and 69.1% in vivo, Chk1 and Chk2 levels were upregulated, CyclinB, CDC25 and CDK1 levels were downregulated.
Keywords:
BEL-7402 cells; In vitro and in vivo; Liver cancer; Mechanism; α-Pinene.
Copyright © 2015 Japanese Pharmacological Society. Production and hosting by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antineoplastic Agents, Phytogenic*
-
Bicyclic Monoterpenes
-
CDC2 Protein Kinase
-
Carcinoma, Hepatocellular / drug therapy
-
Carcinoma, Hepatocellular / genetics
-
Carcinoma, Hepatocellular / pathology*
-
Checkpoint Kinase 1
-
Checkpoint Kinase 2 / metabolism
-
Cyclin B / metabolism
-
Cyclin-Dependent Kinases / metabolism
-
Down-Regulation / drug effects
-
G2 Phase Cell Cycle Checkpoints / genetics*
-
Humans
-
Liver Neoplasms / drug therapy
-
Liver Neoplasms / genetics
-
Liver Neoplasms / pathology*
-
M Phase Cell Cycle Checkpoints / genetics*
-
Mice, Nude
-
Monoterpenes / isolation & purification
-
Monoterpenes / pharmacology*
-
Neoplasm Transplantation
-
Phytotherapy
-
Pinus / chemistry*
-
Plant Oils / chemistry*
-
Protein Kinases / metabolism
-
Tumor Cells, Cultured
-
Up-Regulation / drug effects
Substances
-
Antineoplastic Agents, Phytogenic
-
Bicyclic Monoterpenes
-
Cyclin B
-
Monoterpenes
-
Plant Oils
-
Protein Kinases
-
Checkpoint Kinase 2
-
CHEK1 protein, human
-
Checkpoint Kinase 1
-
Chek1 protein, mouse
-
CDC2 Protein Kinase
-
CDK1 protein, human
-
Cyclin-Dependent Kinases
-
alpha-pinene