Anti-tumor effect of α-pinene on human hepatoma cell lines through inducing G2/M cell cycle arrest

Weiqiang Chen; Ying Liu; Ming Li; Jianwen Mao; Lirong Zhang; Rongbo Huang; Xiaobao Jin; Lianbao Ye

doi:10.1016/j.jphs.2015.01.008

Anti-tumor effect of α-pinene on human hepatoma cell lines through inducing G2/M cell cycle arrest

J Pharmacol Sci. 2015 Mar;127(3):332-8. doi: 10.1016/j.jphs.2015.01.008. Epub 2015 Feb 7.

Authors

Weiqiang Chen¹, Ying Liu¹, Ming Li¹, Jianwen Mao¹, Lirong Zhang¹, Rongbo Huang¹, Xiaobao Jin¹, Lianbao Ye²

Affiliations

¹ School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China.
² Medicinal Chemistry Department, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China. Electronic address: yelb7909@163.com.

PMID: 25837931
DOI: 10.1016/j.jphs.2015.01.008

Abstract

Pine needle oil from crude extract of pine needles has been used as an anti-cancer agent in Traditional Chinese Medicine. The α-pinene is a natural compound isolated from pine needle oil which has been shown anti-cancer activity. In previous study, we found that pine needle oil exhibited significant inhibitory effect on hepatoma carcinoma BEL-7402 cells. In this study, we investigate the inhibition of α-pinene on hepatoma carcinoma BEL-7402 cells in vitro and in vivo and further explore the mechanism. The results show that liver cancer cell growth was inhibited obviously with inhibitory rate of 79.3% in vitro and 69.1% in vivo, Chk1 and Chk2 levels were upregulated, CyclinB, CDC25 and CDK1 levels were downregulated.

Keywords: BEL-7402 cells; In vitro and in vivo; Liver cancer; Mechanism; α-Pinene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents, Phytogenic*
Bicyclic Monoterpenes
CDC2 Protein Kinase
Carcinoma, Hepatocellular / drug therapy
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / pathology*
Checkpoint Kinase 1
Checkpoint Kinase 2 / metabolism
Cyclin B / metabolism
Cyclin-Dependent Kinases / metabolism
Down-Regulation / drug effects
G2 Phase Cell Cycle Checkpoints / genetics*
Humans
Liver Neoplasms / drug therapy
Liver Neoplasms / genetics
Liver Neoplasms / pathology*
M Phase Cell Cycle Checkpoints / genetics*
Mice, Nude
Monoterpenes / isolation & purification
Monoterpenes / pharmacology*
Neoplasm Transplantation
Phytotherapy
Pinus / chemistry*
Plant Oils / chemistry*
Protein Kinases / metabolism
Tumor Cells, Cultured
Up-Regulation / drug effects

Substances

Antineoplastic Agents, Phytogenic
Bicyclic Monoterpenes
Cyclin B
Monoterpenes
Plant Oils
Protein Kinases
Checkpoint Kinase 2
CHEK1 protein, human
Checkpoint Kinase 1
Chek1 protein, mouse
CDC2 Protein Kinase
CDK1 protein, human
Cyclin-Dependent Kinases
alpha-pinene