dbPSP: a curated database for protein phosphorylation sites in prokaryotes

Database (Oxford). 2015 Apr 4;2015:bav031. doi: 10.1093/database/bav031. Print 2015.


As one of the most important post-translational modifications, phosphorylation is highly involved in almost all of biological processes through temporally and spatially modifying substrate proteins. Recently, phosphorylation in prokaryotes attracted much attention for its critical roles in various cellular processes such as signal transduction. Thus, an integrative data resource of the prokaryotic phosphorylation will be useful for further analysis. In this study, we presented a curated database of phosphorylation sites in prokaryotes (dbPSP, Database URL: http://dbpsp.biocuckoo.org) for 96 prokaryotic organisms, which belong to 11 phyla in two domains including bacteria and archaea. From the scientific literature, we manually collected experimentally identified phosphorylation sites on seven types of residues, including serine, threonine, tyrosine, aspartic acid, histidine, cysteine and arginine. In total, the dbPSP database contains 7391 phosphorylation sites in 3750 prokaryotic proteins. With the dataset, the sequence preferences of the phosphorylation sites and functional annotations of the phosphoproteins were analyzed, while the results shows that there were obvious differences among the phosphorylation in bacteria, archaea and eukaryotes. All the phosphorylation sites were annotated with original references and other descriptions in the database, which could be easily accessed through user-friendly website interface including various search and browse options. Taken together, the dbPSP database provides a comprehensive data resource for further studies of protein phosphorylation in prokaryotes. Database URL: http://dbpsp.biocuckoo.org

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Archaea* / genetics
  • Archaea* / metabolism
  • Archaeal Proteins* / genetics
  • Archaeal Proteins* / metabolism
  • Bacteria* / genetics
  • Bacteria* / metabolism
  • Bacterial Proteins* / genetics
  • Bacterial Proteins* / metabolism
  • Databases, Protein*
  • Phosphoproteins* / genetics
  • Phosphoproteins* / metabolism
  • Phosphorylation


  • Archaeal Proteins
  • Bacterial Proteins
  • Phosphoproteins