Catecholamine measurements in urine by high-performance liquid chromatography with amperometric detection--comparison with an autoanalyser fluorescence method

J Chromatogr. 1989 Sep 29;494:53-66. doi: 10.1016/s0378-4347(00)82656-0.

Abstract

In order to validate different methods of measuring urinary catecholamines (norepinephrine, epinephrine and dopamine) in humans, methods based on separation of catecholamines using reversed-phase or cation-exchange high-performance liquid chromatography with electrochemical detection were compared with an autoanalyser-based fluorescence method. Different methods for pre-chromatography sample purification were also studied. For measurements of urinary catecholamines, the reversed-phase-based chromatographic techniques studied were found to give less reliable results than cation-exchange chromatography, even if one of them (Clin Rep Urine Catecholamine Kit) gave almost as precise estimates. The autoanalyser technique yielded good results. It is concluded that cation-exchange chromatography with an appropriate sample work-up procedure (a combination of organic solvent extraction and alumina adsorption) is a reliable and accurate method for analyses of urinary catecholamines.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetates
  • Catecholamines / urine*
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Ion Exchange
  • Electrochemistry
  • Hexanes
  • Humans
  • Male
  • Reagent Kits, Diagnostic
  • Solvents
  • Spectrometry, Fluorescence

Substances

  • Acetates
  • Catecholamines
  • Hexanes
  • Reagent Kits, Diagnostic
  • Solvents
  • ethyl acetate