Chemical characteristics and antithrombotic effect of chondroitin sulfates from sturgeon skull and sturgeon backbone

Carbohydr Polym. 2015 Jun 5;123:454-60. doi: 10.1016/j.carbpol.2015.01.046. Epub 2015 Feb 3.

Abstract

Chondroitin sulfates (CSs) were extracted from sturgeon skull and backbone, and their chemical composition, anticoagulant, anti-platelet and thrombolysis activities were evaluated. The average molecular weights of CS from sturgeon skull and backbone were 38.5kDa and 49.2kDa, respectively. Disaccharide analysis indicated that the sturgeon backbone CS was primarily composed of disaccharide monosulfated in position four of the GalNAc (37.8%) and disaccharide monosulfated in position six of the GalNAc (59.6%) while sturgeon skull CS was primarily composed of nonsulfated disaccharide (74.2%). Sturgeon backbone CS showed stronger antithrombotic effect than sturgeon skull CS. Sturgeon backbone CS could significantly prolong activated partial thromboplastin time (APTT) and thrombin time (TT), inhibited ADP-induced platelet aggregation and dissolved platelet plasma clots in vitro. The results suggested that sturgeon backbone CS can be explored as a functional food with antithrombotic function.

Keywords: Anti-platelet aggregation; Anticoagulant; Chondroitin sulfate; Sturgeon; Thrombolysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anticoagulants / chemistry*
  • Anticoagulants / isolation & purification
  • Anticoagulants / pharmacology
  • Chondroitin Sulfates / chemistry*
  • Chondroitin Sulfates / isolation & purification
  • Chondroitin Sulfates / pharmacology
  • Disaccharides / chemistry
  • Fibrinolytic Agents / chemistry
  • Fibrinolytic Agents / isolation & purification
  • Fibrinolytic Agents / pharmacology
  • Magnetic Resonance Spectroscopy
  • Molecular Weight
  • Partial Thromboplastin Time
  • Platelet Aggregation / drug effects
  • Platelet Aggregation Inhibitors / chemistry
  • Platelet Aggregation Inhibitors / isolation & purification
  • Platelet Aggregation Inhibitors / pharmacology
  • Sharks / metabolism
  • Skull / metabolism*
  • Thrombin Time
  • Vertebrates / metabolism

Substances

  • Anticoagulants
  • Disaccharides
  • Fibrinolytic Agents
  • Platelet Aggregation Inhibitors
  • Chondroitin Sulfates