GPR4 decreases B16F10 melanoma cell spreading and regulates focal adhesion dynamics through the G13/Rho signaling pathway

Calvin R Justus; Li V Yang

doi:10.1016/j.yexcr.2015.03.022

GPR4 decreases B16F10 melanoma cell spreading and regulates focal adhesion dynamics through the G13/Rho signaling pathway

Exp Cell Res. 2015 May 15;334(1):100-13. doi: 10.1016/j.yexcr.2015.03.022. Epub 2015 Apr 4.

Authors

Calvin R Justus¹, Li V Yang²

Affiliations

¹ Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
² Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA; Department of Oncology, Brody School of Medicine, East Carolina University, Greenville, NC, USA; Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC, USA; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, NC, USA. Electronic address: yangl@ecu.edu.

PMID: 25845498
DOI: 10.1016/j.yexcr.2015.03.022

Abstract

The effect of acidosis, a biochemical hallmark of the tumor microenvironment, on cancer progression and metastasis is complex. Both pro- and anti-tumorigenic effects of acidosis have been reported and the acidic microenvironment has been exploited for specific delivery of drugs, imaging agents, and genetic constructs into tumors. In this study we investigate the spreading and focal adhesion of B16F10 melanoma cells that are genetically engineered to overexpress the pH-sensing G protein-coupled receptor GPR4. By using cell attachment assays we found that GPR4 overexpression delayed cell spreading and altered the spatial localization of dynamic focal adhesion complex, such as the localization of phosphorylated focal adhesion kinase (FAK) and paxillin, at acidic pH. The potential G-protein and downstream signaling pathways that are responsible for these effects were also investigated. By using the Rho inhibitor CT04 (C3 transferase), the Rho-associated kinase (ROCK) inhibitors Y27632 and thiazovivin, the myosin light chain kinase (MLCK) inhibitor staurosporine or a G12/13 inhibitory construct, cell spreading was restored whereas the inhibition and activation of the Gq and Gs pathways had little or no effect. Altogether our results indicate that through the G12/13/Rho signaling pathway GPR4 modulates focal adhesion dynamics and reduces cell spreading and membrane ruffling.

Keywords: Acidosis; Cell spreading; Focal adhesion; GPR4; Tumor microenvironment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Adhesion
Cell Line, Tumor
Focal Adhesions / metabolism
GTP-Binding Protein alpha Subunits, G12-G13 / metabolism*
Melanoma / metabolism*
Melanoma / pathology*
Mice
Receptors, G-Protein-Coupled / metabolism*
Signal Transduction*
rho GTP-Binding Proteins / metabolism*

Substances

GPR4 protein, mouse
Receptors, G-Protein-Coupled
GTP-Binding Protein alpha Subunits, G12-G13
rho GTP-Binding Proteins