Open source software for quantification of cell migration, protrusions, and fluorescence intensities

J Cell Biol. 2015 Apr 13;209(1):163-80. doi: 10.1083/jcb.201501081. Epub 2015 Apr 6.

Abstract

Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by lack of access to user-friendly, automated tools. We now describe software designed for the automated quantification of cell migration and morphodynamics. Implemented as a plug-in for the open-source platform, ImageJ, ADAPT is capable of rapid, automated analysis of migration and membrane protrusions, together with associated fluorescently labeled proteins, across multiple cells. We demonstrate the ability of the software by quantifying variations in cell population migration rates on different extracellular matrices. We also show that ADAPT can detect and morphologically profile filopodia. Finally, we have used ADAPT to compile an unbiased description of a "typical" bleb formed at the plasma membrane and quantify the effect of Arp2/3 complex inhibition on bleb retraction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actin-Related Protein 2-3 Complex / metabolism
  • Apoptosis
  • Cell Movement*
  • Cell Shape
  • Cell Tracking
  • HeLa Cells
  • Humans
  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence
  • Pseudopodia / ultrastructure
  • Single-Cell Analysis
  • Software*

Substances

  • Actin-Related Protein 2-3 Complex