Detection of endogenous and food-derived collagen dipeptide prolylhydroxyproline (Pro-Hyp) in allergic contact dermatitis-affected mouse ear

Biosci Biotechnol Biochem. 2015;79(8):1356-61. doi: 10.1080/09168451.2015.1027653. Epub 2015 Apr 7.

Abstract

Generation of collagen dipeptides and deposition of orally administered prolylhydroxyproline (Pro-Hyp) in local inflammatory sites were examined in mice with hapten (2,4-dinitrofluorobenzene)-induced dermatitis in the ear. Pro-Hyp content in the hapten-treated ear was significantly higher in the chronic phase of contact dermatitis than the vehicle control. In contrast, hydroxyprolylglycine contents remained at lower levels in all cases compared to Pro-Hyp. Four hours after the ingestion of [(13)C5,(15)N]Pro and [(13)C5,(15)N]Pro-Hyp, labeled-Pro-Hyp and Pro, respectively, appeared only in the ear with dermatitis. Thus, Pro-Hyp is generated and degraded as part of the rapid synthesis and degradation of collagen in the ear with dermatitis. In addition to the endogenously generated Pro-Hyp, the orally administered Pro-Hyp was deposited in the ears.

Keywords: collagen peptide; dermatitis; hydroxyprolylglycine; inflammation; prolylhydroxyproline.

MeSH terms

  • Administration, Oral
  • Animals
  • Collagen / isolation & purification*
  • Collagen / metabolism
  • Dermatitis, Allergic Contact / etiology
  • Dermatitis, Allergic Contact / metabolism*
  • Dermatitis, Allergic Contact / pathology
  • Dinitrofluorobenzene / toxicity
  • Dipeptides / isolation & purification*
  • Dipeptides / metabolism
  • Ear / pathology
  • Food
  • Inflammation / metabolism*
  • Inflammation / pathology
  • Mice

Substances

  • Dipeptides
  • prolyl-4-hydroxyproline
  • Collagen
  • Dinitrofluorobenzene