Detection of Human Coronavirus 229E in Nasal Washings Using RNA:RNA Hybridisation

J Med Virol. 1989 Sep;29(1):70-3. doi: 10.1002/jmv.1890290113.

Abstract

A method is described for the detection of human coronavirus 229E (HCV 229E) in nasal washings using RNA:RNA filter hybridisation. Volunteers were inoculated with HCV 229E, and daily nasal washings were collected. These washings were then examined for the presence of viral RNA using a single-stranded RNA probe. Nucleic acid hybridisation is shown to be a sensitive technique for the diagnosis of HCV 229E infections.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Coronaviridae / genetics
  • Coronaviridae / isolation & purification*
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • Humans
  • Nasal Cavity / microbiology*
  • Nucleic Acid Hybridization*
  • RNA Probes*

Substances

  • RNA Probes