Cytoprotective and Anti-secretory Effects of Azadiradione Isolated from the Seeds of Azadirachta indica (neem) on Gastric Ulcers in Rat Models

Phytother Res. 2015 Jun;29(6):910-6. doi: 10.1002/ptr.5332. Epub 2015 Apr 8.


Azadirachta indica is well known medicinal plant mentioned in ancient herbal texts. It has been extensively used in Ayurvedic, Unani and Homoeopathic medicine and has become a luminary of modern medicine. As part of our drug discovery program we isolated azadiradione from the ethanolic extract of seeds of A. indica and evaluated for in-vivo antiulcer activity in cold restraint induced gastric ulcer model, aspirin induced gastric ulcer model, alcohol induced gastric ulcers model and pyloric ligation induced ulcer model. Azadiradione exhibited potent antiulcer activity through the inhibition of H+ K+-ATPase (proton pump) activity via its cytoprotective effect and also via its antisecretory effect. This combined effect has valuable potential in the future treatment of peptic ulceration.

Keywords: Azadirachta indica; PGE2; azadiradione; gastric ulcer; proton pump.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Ulcer Agents / isolation & purification
  • Anti-Ulcer Agents / pharmacology*
  • Azadirachta / chemistry*
  • Dinoprostone / chemistry
  • Disease Models, Animal
  • Female
  • Limonins / isolation & purification
  • Limonins / pharmacology*
  • Male
  • Plant Extracts / pharmacology*
  • Plants, Medicinal / chemistry
  • Proton Pump Inhibitors / isolation & purification
  • Proton Pump Inhibitors / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Seeds / chemistry
  • Stomach Ulcer / drug therapy*


  • Anti-Ulcer Agents
  • Limonins
  • Plant Extracts
  • Proton Pump Inhibitors
  • azadiradione
  • Dinoprostone