Positive long-term outcomes from presuckling calcium supplementation in lactating rats and the offspring

Am J Physiol Endocrinol Metab. 2015 Jun 1;308(11):E1010-22. doi: 10.1152/ajpendo.00049.2015. Epub 2015 Apr 7.

Abstract

Adequate dietary calcium intake and the enhanced intestinal calcium absorption in lactating mothers have long been postulated to prevent maternal bone loss and benefit neonatal bone growth. We recently showed that calcium supplementation just before breastfeeding efficiently alleviated lactation-induced bone loss in dams as well as increased milk calcium concentration, which led to higher bone mineral density (BMD) in the newborns. Herein, we further elaborated in detail how presuckling calcium supplements worked in lactating rats and how they benefited bone growth in the offspring. As revealed by bone histomorphometry, presuckling supplement with calcium alone reduced the osteoclast surface and active erosion surface, leading to an increase in trabecular thickness without changes in trabecular separation or number in dams. The beneficial effects of presuckling calcium supplements, particularly the regimen containing glucose and galactose that enhanced intestinal calcium absorption, were found to last for 3 mo postweaning, although it could not restore estrogen-deficient osteopenia induced by ovariectomy. Regarding the neonatal benefits, pups nursed by calcium-supplemented dams exhibited increases in trabecular BMD, which could be observed even at the age of 27 wk. Bone elongation was also greater in pups of calcium-supplemented dams, which was due possibly to accelerated growth plate chondrocyte turnover. It could be concluded that calcium supplements markedly diminished the lactation-induced osteopenia in dams and positively affected BMD and bone elongation in growing rats. Therefore, presuckling calcium supplementation in lactating mothers is an effective strategy for promoting a long-lasting high bone density for both mother and the offspring.

Keywords: bone elongation; bone histomorphometry; growth plate; lactation-induced osteoporosis; oral calcium supplement.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Newborn
  • Animals, Suckling
  • Bone Density / drug effects
  • Bone Development / drug effects*
  • Calcium, Dietary / pharmacology*
  • Dietary Supplements
  • Female
  • Lactation / drug effects*
  • Lactation / metabolism
  • Male
  • Maternal Nutritional Physiological Phenomena* / drug effects
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors

Substances

  • Calcium, Dietary