Lactobacilli vaginal colonisation after oral consumption of Respecta(®) complex: a randomised controlled pilot study

Arch Gynecol Obstet. 2015 Oct;292(4):861-7. doi: 10.1007/s00404-015-3711-4. Epub 2015 Apr 9.


Purpose: The aim of the current pilot study was to determine if oral consumption of a combination of two probiotics; L. acidophilus La-14, L. rhamnosus HN001, and bovine lactoferrin (Respecta(®) complex), would lead to the detection with molecular techniques of the consumed probiotic strains in the vagina.

Methods: Healthy volunteers (40) consumed the study product twice daily for 2 weeks. Vaginal swabs were collected at 0, 1, 2 and 3 weeks and analysed for the consumed organisms by qPCR.

Results: Vaginal L. rhamnosus and L. acidophilus levels were significantly increased on days 14 and 21. On days 14 and 21 a significant number of women had increased levels of vaginal L. acidophilus and on days 7 and 21 a significant number of women had increased levels of vaginal L. rhamnosus.

Conclusions: Consumption of L. acidophilus La-14, L. rhamnosus HN001 in combination with bovine lactoferrin leads to vaginal detection; even 1 week after consumption was stopped. This provides a basis for future studies on urogenital tract health.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Adolescent
  • Adult
  • Animals
  • Anti-Infective Agents / administration & dosage*
  • Cattle
  • Colony Count, Microbial
  • Double-Blind Method
  • Female
  • Humans
  • Lactobacillus / drug effects
  • Lactobacillus / genetics*
  • Lactobacillus / isolation & purification*
  • Lactobacillus acidophilus / genetics
  • Lactobacillus acidophilus / isolation & purification
  • Lactobacillus rhamnosus / genetics
  • Lactobacillus rhamnosus / isolation & purification
  • Lactoferrin / administration & dosage*
  • Pilot Projects
  • Polymerase Chain Reaction
  • Probiotics / administration & dosage*
  • Vagina / microbiology*
  • Vaginal Smears / methods


  • Anti-Infective Agents
  • Lactoferrin