Multidimensional proteomics for cell biology

Nat Rev Mol Cell Biol. 2015 May;16(5):269-80. doi: 10.1038/nrm3970. Epub 2015 Apr 10.

Abstract

The proteome is a dynamic system in which each protein has interconnected properties - dimensions - that together contribute to the phenotype of a cell. Measuring these properties has proved challenging owing to their diversity and dynamic nature. Advances in mass spectrometry-based proteomics now enable the measurement of multiple properties for thousands of proteins, including their abundance, isoform expression, turnover rate, subcellular localization, post-translational modifications and interactions. Complementing these experimental developments are new data analysis, integration and visualization tools as well as data-sharing resources. Together, these advances in the multidimensional analysis of the proteome are transforming our understanding of various cellular and physiological processes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Physiological Phenomena*
  • Cytological Techniques
  • Databases, Protein
  • Humans
  • Mass Spectrometry
  • Organ Specificity
  • Protein Processing, Post-Translational
  • Proteins / analysis
  • Proteins / metabolism
  • Proteomics / methods*

Substances

  • Proteins