Cordyceps sinensis polysaccharide inhibits PDGF-BB-induced inflammation and ROS production in human mesangial cells

Carbohydr Polym. 2015 Jul 10;125:135-45. doi: 10.1016/j.carbpol.2015.02.012. Epub 2015 Feb 19.

Abstract

CPS-F, a polysaccharide derived from Cordyceps sinensis, is a potential anti-inflammatory and anti-oxidative agent. We demonstrated that CPS-F not only inhibits platelet-derived growth factor BB (PDGF-BB)-induced intracellular reactive oxygen species (ROS) generation, and up-regulation of tumor necrosis factor-α (TNF-α), TNF-α receptor 1 (TNFR1), and monocyte chemotactic protein-1 (MCP-1), but also acts synergistically in combination with MAPK/ERK inhibitor U0126 and PI3K/Akt inhibitor LY294002. Additionally, up-regulation of pro-inflammatory factors was reversed by use of a combination of CPS-F and NADPH oxidase (NOX) inhibitor diphenyleneiodonium chloride (DPI) or silencing of NOX1. Furthermore, CPS-F prevents the PDGF receptor β (PDGFRβ) promoter activity induced by PDGF-BB in transfected cells and ameliorates increased levels of TNF-α, TNFR1, and MCP-1 when PDGFRβ is silenced, thereby suggesting that CPS-F possesses a bidirectional regulatory function. Our findings suggest CPS-F may exert its therapeutic effect for the treatment of glomerulonephritis related to human mesangial cells (HMCs) through the ERK1/2/Akt pathways.

Keywords: Cordyceps sinensis; Inflammation; NADPH oxidase; Polysaccharide; Reactive oxygen species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / pharmacology*
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Becaplermin
  • Butadienes / pharmacology
  • Cell Line
  • Chemokine CCL2 / genetics
  • Chemokine CCL2 / metabolism
  • Chromones / pharmacology
  • Cordyceps / chemistry*
  • Fungal Polysaccharides / chemistry
  • Fungal Polysaccharides / pharmacology*
  • Humans
  • Mesangial Cells / drug effects*
  • Mesangial Cells / metabolism
  • Morpholines / pharmacology
  • NADPH Oxidase 1
  • NADPH Oxidases / genetics
  • NADPH Oxidases / metabolism
  • Nitriles / pharmacology
  • Onium Compounds / pharmacology
  • Protein Kinase Inhibitors / pharmacology
  • Proto-Oncogene Proteins c-sis / metabolism*
  • Reactive Oxygen Species / metabolism
  • Receptors, Tumor Necrosis Factor / genetics
  • Receptors, Tumor Necrosis Factor / metabolism
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anti-Inflammatory Agents
  • Antioxidants
  • Butadienes
  • CCL2 protein, human
  • Chemokine CCL2
  • Chromones
  • Fungal Polysaccharides
  • Morpholines
  • Nitriles
  • Onium Compounds
  • Protein Kinase Inhibitors
  • Proto-Oncogene Proteins c-sis
  • Reactive Oxygen Species
  • Receptors, Tumor Necrosis Factor
  • Tumor Necrosis Factor-alpha
  • U 0126
  • Becaplermin
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • diphenyleneiodonium
  • NADPH Oxidase 1
  • NADPH Oxidases
  • NOX1 protein, human