Label-free kinetic analysis of an antibody-antigen interaction using biolayer interferometry

Methods Mol Biol. 2015:1278:165-82. doi: 10.1007/978-1-4939-2425-7_10.


Biolayer Interferometry (BLI) is a powerful technique that enables direct measurement of biomolecular interactions in real time without the need for labeled reagents. Here we describe the analysis of a high-affinity binding interaction between a monoclonal antibody and purified antigen using BLI. A simple Dip-and-Read™ format in which biosensors are dipped into microplate wells containing purified or complex samples provides a highly parallel, user-friendly technique to study molecular interactions. A rapid rise in publications citing the use of BLI technology in a wide range of applications, from biopharmaceutical discovery to infectious diseases monitoring, suggests broad utility of this technology in the life sciences.

MeSH terms

  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / immunology
  • Antigens / chemistry*
  • Antigens / immunology
  • Biosensing Techniques*
  • Interferometry
  • Kinetics
  • Protein Interaction Mapping
  • Staining and Labeling


  • Antibodies, Monoclonal
  • Antigens