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. 2015;1278:323-7.
doi: 10.1007/978-1-4939-2425-7_19.

Fluorescence Polarization Assay to Quantify Protein-Protein Interactions: An Update

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Fluorescence Polarization Assay to Quantify Protein-Protein Interactions: An Update

Ronald T Raines. Methods Mol Biol. .

Abstract

A fluorescence polarization assay can be used to evaluate the strength of a protein-protein interaction. A green fluorescent protein variant is fused to one of the protein partners. The formation of a complex is then deduced from an increase in fluorescence polarization, and the equilibrium dissociation constant of the complex is determined in a homogeneous aqueous environment. The assay is demonstrated by using the interaction of the S-protein and S-peptide fragments of ribonuclease A as a case study.

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