Pulsed-field gel electrophoresis for disease monitoring and control

John Besser

doi:10.1007/978-1-4939-2599-5_1

Pulsed-field gel electrophoresis for disease monitoring and control

Methods Mol Biol. 2015:1301:3-7. doi: 10.1007/978-1-4939-2599-5_1.

Author

John Besser¹

Affiliation

¹ Enteric Diseases Laboratory Branch, NCEZID/DFWED, MS C03, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, 30333, USA, JBesser@cdc.gov.

PMID: 25862043
DOI: 10.1007/978-1-4939-2599-5_1

Abstract

Pulsed-field gel electrophoresis (PFGE) is the "gold standard" for molecular subtyping and has significant applications in disease monitoring and control programs. This chapter discusses the advantages of PFGE in light of developing technologies such as whole-genome sequencing.

MeSH terms

Bacteria / classification
Bacteria / genetics*
Bacteria / pathogenicity
Bacterial Typing Techniques / methods*
Electrophoresis, Gel, Pulsed-Field / methods*
Food Microbiology
Humans
Infections / microbiology*
Infections / pathology